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A common growth medium in biochemistry & microbiology labs is potato dextrose agar (PDA). Yeasts, moulds, & bacteria are just a few of the many microorganisms that can be grown in this nutrient-rich media.
CHEMICAL PRODUCT IDENTIFICATION
- Product Name: Potato Dextrose Agar (PDA).
- Signal Phrase: N/A.
HAZARDS IDENTIFICATION
- According to the GHS classification for the Hazard Communication Standard, this chemical is not deemed dangerous.
- All laboratory substances should be handled with care.
- This substance helps the growth of pathogens(disease-causing microorganisms).
- When cultivating, use a good aseptic procedure.
- Sterilization is required before disposing of cultures.
COMPOSITION, INFORMATION ON INGREDIENTS
Component Name | CAS Number | Formula | Formula Weight | Concentration |
---|---|---|---|---|
Agar, bacteriological grade | 9002-18-0 | Unspecified | Unspecified | 38% |
Dextrose, anhydrous | 492-62-6 | C6H12O6 | 180.16 | 52% |
Potato extract | nil established | Unspecified | Unspecified | 10% |
FIRST-AID MEASURES
- If you feel unwell, contact a doctor.
- If inhaled:Â Remove the victim to fresh air & keep them at rest in a breathing-friendly position.
- If in the eyes:Â Carefully rinse for a few minutes with water. If contact lenses are present and simple to remove, do so. Rinsing continues.
- If on the skin:Â Thoroughly rinse with water.
- If Swallowed: Rinse mouth if swallowed. If you feel unwell, contact a doctor.
FIRE-FIGHTING MEASURES
- It is non-flammable (solid).
- Toxic gases could be released during decomposition when heated.
- Use a tri-class dry chemical fire extinguisher in the event of a fire.
ACCIDENTAL RELEASE MEASURES
- Clean up any spills & it should autoclave if the medium has already been used for culturing.
- Dispose of it in the trash.
HANDLING & STORAGE
- Store in a chemical safety bag in a cold, dry location.
EXPOSURE CONTROLS, PERSONAL PROTECTION
- Avoid making eye contact.
- Use sanitary procedures when handling culture media.
- After handling, carefully wash your hands.
PHYSICAL & CHEMICAL PROPERTIES
- White to off-white grains or powder.
- sour mash aroma.
- Solubility:Â uses hot water to create a gel.
- Biological application:Â uses hot water to create a gel.
- Getting ready cultural media Per litre of water, combine 39 gm. One must perform sterilization before use.
- The medium has a pH range of: 6.0 to 7.5.
STABILITY & REACTIVITY
- It is considered a non-hazardous substance.
- Good shelf life if properly stored.
TOXICOLOGICAL INFORMATION
- Acute effects: N.A
- Chronic effects: N.A
- Target organs: N.A
- N.A. for ORL-RAT LD50.
- LC50 for IHL-RAT: N.A.
- LD50 for SKN-RBT: N.A.
ECOLOGICAL INFORMATION
- Not Available.
DISPOSAL CONSIDERATIONS
- Dispose of in accordance with local, state, & federal rules or laws.
TRANSPORT INFORMATION
- Name of shipment: Unrestricted. Risk category: N/A. UN identifier: N/A.
REGULATORY INFORMATION
- Not included.
OTHER INFORMATION
This Safety Data Sheet (SDS) is intended as a guide & is based on data & tests that are likely to be reliable. I don’t guarantee the data’s correctness or completeness & I’m not responsible for any resulting losses. The information is only being provided for your review, research, & verification. I DO NOT ASSUME RESPONSIBILITY AND EXPRESSLY DISCLAIM LIABILITY FOR LOSS, DAMAGE OR EXPENSE RESULTING FROM OR IN ANY WAY CONNECTED WITH THE HANDLING, STORAGE, USE OR DISPOSAL OF THIS PRODUCT FOR THE FOREGOING AND OTHER REASONS.
CONCLUSION
PDA is made up of agar to act as a solidifier, dextrose, & potato infusion. In microbiological & biochemical laboratories, potato dextrose agar is a common growth medium, but it must be handled & stored carefully to avoid contamination and potential health risks. To stop the growth & spread of microbes, PPE like lab coats, gloves, & face masks should be worn when handling the medium. Any used PDA should also be disinfected before disposal. To guarantee a safe working environment, correct handling guidelines & safety precautions should always be observed during the laboratory study.
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