Eosin-Methylene Blue Agar SDS

According to GHS classifications for the Hazard Communication Standard, this chemical is not deemed dangerous. All laboratory substances should be handled with care.
Pathogenic microbes may be able to grow on this material. When cultivating, use suitable aseptic procedures. Sterilization is required before disposing of cultures.

COMPOSITION, INFORMATION ON INGREDIENTS

Component Name	CAS Number	Formula	Formula Weight	Concentration
Agar, bacteriological grade	9002-18-0	Unspecified	Unspecified	37.5%
Peptone (source: bovine)	91079-38-8	Unspecified	Unspecified	27.8%
Lactose monohydrate	64044-51-5	C12H22O11•H2O	360.31	13.9%
Sucrose	57-50-1	C12H22O11	342.31	13.9%
Potassium phosphate dibasic	7758-11-4	K2HPO4	174.18	5.5%
Eosin Y	17372-87-1	C20H6Br4Na2O5	691.91	1.1%
Methylene blue trihydrate	7220-79-3	C16H18ClN3S•3H2O	373.90	0.2%

Synonyms: EMB Agar.

FIRST-AID MEASURES

If you feel unwell, contact a doctor.
If inhaled: Remove the victim to fresh air and keep them at rest in a breathing-friendly position.
If in eyes: Rinse carefully for a few minutes with water. If contact lenses are present and simple to remove, do so. Rinsing continues.
If on the skin: Wash with plenty of water.
If swallowed: Rinse mouth. Call a doctor if you feel unwell.

FIRE-FIGHTING MEASURES

Nonflammable solid.
When heated to decomposition, it may emit toxic fumes.
In case of fire: Use a tri-class dry chemical fire extinguisher.

ACCIDENTAL RELEASE MEASURES

Clean up any spills & sanitize any equipment that has already been used for culturing. Place it in the garbage. For additional details, refer to Sections 8 and 13.

HANDLING & STORAGE

Moisture sensitive. Store in a cool, dry place within a chemical safety bag.

EXPOSURE CONTROLS, PERSONAL PROTECTION

Avoid making eye contact. When working with culture media, employ sterile procedures. After handling, carefully wash your hands.

PHYSICAL & CHEMICAL PROPERTIES

Appearance: Pink/violet powder. Malt-like odor.
Soluble: Forms a gel with boiling water.
Biological use: Differential bacterial media (gram-negative bacteria).
To prepare culture media: Mix 36 grams per liter of water. Sterilize before use.

STABILITY & REACTIVITY

The product that is stable & safe.
Good shelf life if properly stored.

TOXICOLOGICAL INFORMATION

Acute effects: N.A.
Chronic effects: N.A.
Target organs: N.A.
N.A. for ORL-RAT LD50.
LC50 for IHL-RAT: N.A.
LD50 for SKN-RBT: N.A.

ECOLOGICAL INFORMATION

Data is not yet available.

DISPOSAL CONSIDERATIONS

Please check all applicable federal, state, & local laws before moving forward.
Disposal Method Sugession: Sterilize prior to disposal.

TRANSPORT INFORMATION

Shipping name: Not regulated. Hazard class: N/A. UN number: N/A.

REGULATORY INFORMATION

Not listed.

OTHER INFORMATION

This Eosin-Methylene Blue Agar SDS is intended as a piece of advice & is based on data & tests that are likely to be reliable. I don’t guarantee the data’s correctness or completeness & I’m not responsible for any resulting losses. The information is only being provided for your review, research, & verification. I DO NOT ASSUME RESPONSIBILITY AND EXPRESSLY DISCLAIM LIABILITY FOR LOSS, DAMAGE, OR EXPENSE RESULTING FROM OR IN ANY WAY CONNECTED WITH THE HANDLING, STORAGE, USE, OR DISPOSAL OF THIS PRODUCT FOR THE FOREGOING AND OTHER REASONS.

CONCLUSION

Eosin-methylene blue agar is a valuable tool for the identification & differentiation of pathogenic microorganisms. This differential media has been used for many years as a reliable & accurate method to identify bacteria that can cause human illness. The combination of the two dyes eosin & methylene blue allows for the detection of colonies with different morphologies and the identification of different bacterial species. EMB agar has been used to identify bacteria such as Salmonella, Shigella, Escherichia coli, and Staphylococcus aureus. The accuracy & reliability of this agar provide a significant advantage in the identification of pathogens and can be used in both clinical & research settings.

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