Cultivating the Perfect Cells: Czapek-Dox-Agar SDS

A typical growing media used in biochemistry & microbiology labs is Czapek-Dox Agar. It is used to cultivate & identify a variety of yeasts, moulds, & fungi, particularly filamentous fungi.

HAZARDS IDENTIFICATION

According to CLP Classification-Regulation, It is not a dangerous compound.
Considering it is a chemical which is usually placed along with the other relative chemicals then it is advised to follow the laboratory chemicals protocol while handling this or any other product.

COMPOSITION, INFORMATION ON INGREDIENTS

Ingredients	Quantity	Functionality
Sucrose	30 gm	Source of Carbon
Sodium Nitrate	2.0 gm	Source of Nitrogen
Dipotassium Phosphate	1.0 gm	Buffers the Medium
Magnesium Sulfate	0.5 gm	Source of essential Ions
Potassium Chloride	0.5 gm	Source of essential Ions
Ferrous Sulfate	0.01 gm	Source of essential Ions
Agar	15.00 gm	Solidifying Agent
Distilled Water	1 L (1000 ml)	for preparing the Solution

FIRST-AID MEASURES

If you feel unwell, contact a doctor.
If inhaled: Remove the victim to fresh air & keep them at rest in a breathing-friendly position.
If in the eyes: Carefully rinse for a few minutes with water. If contact lenses are present & simply possible to remove, do so. Rinsing continues.
If on the Skin: Wash your hands thoroughly using soap & water. Remove any contaminated garments right away, & if irritation persists then take medical attention.
Most significant immediate & delayed signs or symptoms are none.

ACCIDENTAL RELEASE MEASURES

Under the anticipated conditions of usage, no special preventative health measures should be required.
Wear the proper personal protective equipment (PPE), such as a lab coat, safety glasses, & gloves, as directed by your lab’s protocol.
Clean up the spill.
Avoid dust formation.
Clean up with inert absorbent material, then discard everything polluted as garbage.
Avoid releasing this into the environment.

HANDLING & STORAGE

Avert breathing in dust. Eliminate contact with the skin, eyes, & clothing.
Store in a dry, cold place. Keep the container firmly shut.
For recommended storage temperature always follow the product labelling.
Store in a firmly closed container at or below 8°C.

EXPOSURE CONTROLS, PERSONAL PROTECTION

No chemicals that have occupational exposure limit values are present.
Engineering Controls: General industrial hygiene best practice; Keep skin, eyes, & clothing away from touch. Before & after handling the item, wash your hands thoroughly.
Wear appropriate eye protection, gloves, & clothes.
After handling, carefully wash your hands.
Controls for environmental exposure: Don’t pour into drains.

PHYSICAL & CHEMICAL PROPERTIES

Available Form: Powder.
Prepared Appearance: Light yellow to amber-coloured, slightly opalescent gel.
pH: 7.3 ± 0.2.

STABILITY & REACTIVITY

In terms of Reactivity: it is stable under suggested storage or transport conditions.
This chemical is stable under recommender storage conditions.
Hazardous reactions won’t happen while being transported or stored normally.
To maintain the stability of your product, keep away from heat & moisture.
Hazardous decomposition products: If utilised as directed, there is no decomposition issue.

TOXICOLOGICAL INFORMATION

Acute toxicity: No data available.
Skin corrosion/irritation: No data available.
Aspiration hazard: No data available
Reproductive toxicity: No data available.
Specific target organ toxicity- single exposure: No data available.
Serious eye damage/eye irritation: No data available.
Respiratory or skin sensitization: No data available.
Carcinogenicity IARC: No data available.
Germ cell mutagenicity: No data available.

Potential negative health effects:
Inhalation: If inhaled, it might be irritating.
On the Skin: No effects of irritation.
Into the Eyes: No effects of irritation.
Ingestion: It may be harmful.
Sensitization Effects: No known effects of sensitization. Individuals who are sensitive may develop allergies after repeated exposure.

ECOLOGICAL INFORMATION

There are no data on toxicity.
No information is known on persistence & degradation.
There are no data available on bioaccumulative potential.
There are no data on soil mobility.

DISPOSAL CONSIDERATIONS

The user is made aware of the possibility of local or federal rules regarding disposal.
Handle unclean containers with the same care as the goods.
Before disposing of the prepared material & contaminated containers, make sure they have been autoclaved before discard.

TRANSPORT INFORMATION

UN Number: Not Classified.
UN appropriate shipping name: International regulations on the carriage of dangerous products (IMDG, IATA, ADA/RID) do not apply to this product.
Hazard class(es) for Transport: Not hazardous.
User-specific safety precautions: Keep the item in the designated storage place.

REGULATORY INFORMATION

All modes of transportation are secure.

OTHER INFORMATION

This essential information on Czapek-Dox-Agar SDS is intended as a piece of advice & is based on data & tests that are likely to be reliable. I don’t guarantee the data’s correctness or completeness & I’m not responsible for any resulting losses. The information is only being provided for your review, research, & verification. I DO NOT ASSUME RESPONSIBILITY AND EXPRESSLY DISCLAIM LIABILITY FOR LOSS, DAMAGE OR EXPENSE RESULTING FROM OR IN ANY WAY CONNECTED WITH THE HANDLING, STORAGE, USE OR DISPOSAL OF THIS PRODUCT FOR THE FOREGOING AND OTHER REASONS.

CONCLUSION

Follow the essential information mentioned above in Czapek-Dox-Agar SDS, A special kind of growth media called Czapek-Dox Agar is frequently used in microbiological & biochemistry laboratories to cultivate & identify different kinds of fungi, yeasts, & moulds. Although it poses no physical risks, it should be handled carefully to avoid contamination & any health risks. To stop the growth & spread of microbes, PPE such as lab coats, gloves, & face masks should be worn while handling the medium. Any spent Czapek-Dox Agar should also be sterilised before disposal. To guarantee a safe working environment, suitable handling techniques & safety precautions must always be followed during laboratory experiments.

It is recommended to use Czapek-Dox Agar when growing bacteria & fungi that can utilise inorganic nitrogen. It is advised that you use it in high-quality processes for growing saprophytic fungus & soil bacteria. The inorganic source of nitrogen is nitrate, while the inorganic source of carbon is sucrose. Oxoid Czapek Dox Agar, a variant of Czapek Dox Agar, comprises magnesium glycerophosphate & potassium sulphate in place of the originals magnesium sulphate & potassium phosphate. The precipitation of magnesium phosphate is stopped by this change. Additionally, Candida albicans may produce chlamydospores on this medium with great success.

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CHEMICAL PRODUCT IDENTIFICATION